PHARMACEUTICAL INDUSTRY & PHARMACOLOGY STUDIES
Pharmacology studies (ADME)
C.RIS Pharma performs exploratory studies on your drug candidate to validate its mechanism of action and measure its activity in vivo through different bioanalytical methods.
For pharmacokinetic and toxicokinetic studies, samples can be analysed by ELISA or HPLC coupled with UV, VIS and/or MS detectors. Analytical methods can be provided by the Sponsor or developed in our laboratories depending on the characteristics of the drug. The focus lies on providing data to further understand the behaviour and fate of the new compound in a living organism.
C.RIS Pharma also examines the biodistribution and bioavailability of your drug candidate by analysing its presence and persistence in targeted or non-targeted organs. Quantitative analyses of the biological samples can be performed by HPLC-MS/MS, flow cytometry, ELISA or Q-PCR-ALU methods according to the nature of the test substance. For various reasons, a qualitative analysis may be more suited, and immunohistochemistry and FISH technics can be conducted.
Assessing and characterising the immune response is crucial in all drug developing. A wide range of techniques and expertise are available from C.RIS Pharma to monitor the induced immune response. The cytokine release and antibody production are measured in biological samples and cell culture supernatant using the ELISA method. For more precise information on the immune response of cells, ELISpot method and flow cytometry are used. Histopathology and immunohistochemistry services are also available to study the effect of the drug on the tissue.
Metabolism assays
C.RIS Pharma has designed in vitro and in vivo metabolism assays to help you gain understanding of the metabolic profile of your drug. In vitro, drug metabolism is studied using Liver Metabolic Fractions. We can also evaluate the effects of the treatment on hepatic metabolism by analysing hepatic enzyme activities (P450, …).
In vivo, we can determine the drug metabolism by HPLC-MS/MS.
Safety Pharmacology
Safety pharmacology studies are presently under development in our company. The aim is to assess the vital functions of animals after drug exposure in toxicology studies. The cardiovascular system, the respiratory function and the central nervous system (CNS) will be monitored through different tests.
For general behaviour, we currently practise the FOB test in our lab.
